A highly carbapenem-resistant Pseudomonas aeruginosa isolate with a novel blaVIM-4/blaP1b integron overexpresses two efflux pumps and lacks OprD
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چکیده
منابع مشابه
Isolation of an integron-borne blaVIM-4 type metallo-beta-lactamase gene from a carbapenem-resistant Pseudomonas aeruginosa clinical isolate in Hungary.
The first integron-borne metallo-beta-lactamase gene was isolated in Hungary. The bla(VIM-4) gene is located on a class 1 integron that also carries a novel bla(OXA)-like gene. The integron is harbored by a serotype O12 Pseudomonas aeruginosa strain and shows high structural similarity to integrons isolated in Greece and Poland.
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While meropenem MICs were strongly influenced by the presence or absence of the MexAB-OprM efflux pump in both OprD-proficient and -deficient strain backgrounds, MICs of imipenem and of ER-35786 remained unchanged, demonstrating that meropenem is a substrate of MexAB-OprM but not imipenem and ER-35786. In vitro, all three carbapenems selected loss of OprD as a first mechanism of resistance. How...
متن کاملInterplay of efflux system, ampC, and oprD expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates.
Carbapenems are important agents for the therapy of infections due to multidrug-resistant Pseudomonas aeruginosa; the development of carbapenem resistance hampers effective therapeutic options. To assess the mechanisms leading to resistance, 33 clinical isolates with differing degrees of carbapenem susceptibility were analyzed for the expression of the chromosomal beta-lactamase (ampC), the por...
متن کاملCloning and Characterization of blaVIM, a New Integron-Borne Metallo-b-Lactamase Gene from a Pseudomonas aeruginosa Clinical Isolate
LAURA LAURETTI, MARIA LETIZIA RICCIO, ANNARITA MAZZARIOL, GIUSEPPE CORNAGLIA, GIANFRANCO AMICOSANTE, ROBERTA FONTANA, AND GIAN MARIA ROSSOLINI* Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100-Siena, Istituto di Microbiologia, Università di Verona, 37134-Verona, and Dipartimento di Scienze e Tecnologie Biomediche e Biometria, Università dell’Aquila, 6710...
متن کاملCloning and characterization of blaVIM, a new integron-borne metallo-beta-lactamase gene from a Pseudomonas aeruginosa clinical isolate.
Production of a metallo-beta-lactamase activity was detected in a carbapenem-resistant Pseudomonas aeruginosa clinical isolate (isolate VR-143/97) from an Italian inpatient at the Verona University Hospital (northern Italy). The metallo-beta-lactamase determinant was isolated from a genomic library of VR-143/97, constructed in an Escherichia coli plasmid vector, by screening for clones with red...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 2007
ISSN: 1460-2091,0305-7453
DOI: 10.1093/jac/dkm126